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KMID : 0382619870070010407
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Hanyang Journal of Medicine
1987 Volume.7 No. 1 p.407 ~ p.416
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Changes in Nucleic Acid Contents and Nuclease Activities in Lung Cancer Tissues
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Abstract
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Nucleic acid and protein contents and activities of nucleic acid degrading enzymes in lung cancer tissues were determined and were compared with those in normal lung tissues to understand the abnormalities of nucleic acid metabolism in lung tissues. Nucleic acid degrading enzymes and proteins in lung tissues were isolated and frac. tionated by a DEAE-cellulose column chromatography to find out whether the enzymes and proteins specific to lung cancer might be present in the cancer tissues.
Nucleic acid contents and deoxyribonuclease (DNase) activity were significantly higher in lung cancer tissues than in normal lung tissues. The positive rates of DNA and RNA contents and DNase activity as markers for lung cancer were high suggesting that these parameters could be used as diagnostic markers. Ribonuclease (RNase) activities in lung cancer tissues and in serum of patients with lung cancer were significantly increased, but positive rate of serum RNase activity as a marker for lung cancer was low. DEAE-cellulose column chromatographical analyses for RNases and proteins in lung cancer tissues revealed that peak I RNase appeared to be the enzyme specific to lung cancer and that non-RNase peak VIII protein observed in normal lung tissues disappeared in lung cancer tissues. The significance of the abnormal chromatographical pattern in lung cancer has not clearly been understood.
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